- Although diallel designs are difficult with forest trees, because of the problems of quickly completing all possible combinations of control crosses, they have been attempted by several workers (2, 3, 23, 107, 156). (Libby, Stettler, Seitz, 1969)
- Restriction fragment length polymorphism (RFLP) analysis and gene iso-lation are significantly more difficult with large genomes. (Neale et al., 1994)
- Accurate estimation of relatedness requires many marker loci and is, therefore, difficult with microsatellites. (Bijma, 2010)
- Vegetative propagation of juvenile material is relatively easy in most tree species, but rooting becomes increasingly difficult with increasing age. (White, Adams, Neale, 2007)
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